All tests should be conducted under optimal conditions for insect development and with sufficient light and nutrients to maintain plant quality. The temperature regime selected for insect development will also be suitable for the host plant.
Tests must be replicated. A minimum of three replications is normal. For choice tests, a different combination of plants species should be used in each replicate. The exposure period should consist of a minimum of two days as there may be diel rhythms of activity. Also, insects may not begin to feed or lay on a less acceptable host until a period of deprivation has occurred.
