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Large amounts of spores need to be produced, using a suitable culture technique, for release in the field. The technique may involve culture on the weed host or use of artificial media
(Auld 1997b). Depending on circumstance, these spores might be used in the fresh state or collected and stored for a period prior to release. Drying and refrigeration at
4ºC or in liquid nitrogen is a suitable storage method.
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Pathogens may be cultured on the host.
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